Coding

Part:BBa_K258101

Designed by: Cihan Tastan   Group: iGEM09_METU-Gene   (2009-10-10)


PrtE domain of ABC transporter of Erwinia chrysanthemi

prtE, codes for a polypeptide of 448 amino acid residues (predicted Mr = 49.5 kd) which is probably initiated at an ATG codon located 14 bp downstream from the prtD gene stop codon TGA. It is preceded 11 bp upstream by a GGGA RBS consensus sequence.The hydropathy profile of the PrtE protein shows at least one hydrophobic domain with the sequence (residues 30 to 60) which might be a transmembrane segment. No signal peptide is found at the N-terminus, which is hydrophilic

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1136
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1136
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1136
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1136
    Illegal AgeI site found at 1270
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None